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KMID : 0604520080340030189
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Journal of the Society of Cosmetic Scientists of Korea
2008 Volume.34 No. 3 p.189 ~ p.200
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Antioxidative Activity and Component Analysis of Quercus glauca Leaf Extracts
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Yang Hee-Jung
Ahn You-Jin
Kim Jae-Hyun
Park Soo-Nam
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Abstract
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In this study, the antioxidative effects, inhibitory effects on elastase, and components of Quercus glauca extracts were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity of extract I fractions of Quercus glauca leaf was in the order: 50% ethanol extract < ethyl acetate fraction < deglycosylated flavonoid aglycone fraction . Reactive oxygen species (ROS) scavenging activities of some Quercus glauca leaf extracts on ROS generated in system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activity was 50% ethanol extract < deglycosylated flavonoid aglycone fraction < ethyl acetate fraction . Ethyl acetate fraction showed the most prominent scavenging activity. The protective effects of extract / fractions of Quercus glauca leaf on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Quercus glauca leaf extracts suppressed photohemolysis in a dose dependent manner, particularly deglycosylated flavonoid aglycone fraction exhibited the most prominent celluar protective effect , 398.67 min at ). Aglycone fractions obtained from the deglycosylation reaction of ethyl acetate fraction among the Quercus glauca leaf extracts, showed 2 bands in TLC and 2 peaks in HPLC experiments (360 nm) as well. Two components were identified as quercetin (55.77%), and kaempferol (44.23 %). TLC chromatogram of ethyl acetate fraction of Quercus glauca leaf extracts revealed 6 bands , Among them, isoquercitrin (QG3), hyperin (QG4), and rutin (QG6) were identified. The inhibitory effect of aglycone fraction on tyrosinase and elastase was high. These results indicate that extract / fractions of Quercus glauca can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging and other ROS, and protect cellular membranes against ROS. And component analysis of Quercus glauca leaf extract and inhibitory activity on tyeisinase and elastase of the aglycone fraction could be applicable to new functional cosmetics.
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KEYWORD
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quercus glauca, flavonoid, antioxidative activity, tyrosinase, cosmetics
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